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In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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在纳米粒子中的可触发原细胞捕获微滴

Ning Gao1,2, Can Xu2, Zhuping Yin2

  • 1Max Planck-Bristol Centre for Minimal Biology, University of Bristol, Bristol BS8 1TS, U.K.

Journal of the American Chemical Society
|February 22, 2022
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种具有刺激反应的细胞膜的细胞模型. 这种人工细胞系统可以控制其他原细胞的吸收,为合成细胞群落和微型输送系统铺平了道路.

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科学领域:

  • 仿生化学
  • 合成生物学
  • 材料科学

背景情况:

  • 控制人工细胞团队对于开发更高层次的细胞模拟行为至关重要.
  • 现有的模型缺乏对分区间相互作用和物质交换的精确控制.

研究的目的:

  • 设计一个带有刺激反应膜的细胞模型来控制细胞吸收.
  • 在合成原细胞群体中展示可触发的跨膜吸收机制.
  • 探索基于表面特性和协同捕获的原细胞分类.

主要方法:

  • 具有分子拥挤的协细胞内部的化原细胞模型的开发.
  • 使用金 (Au) /聚乙烯糖醇 (PEG) 纳米颗粒构建响应刺激的膜.
  • 通过光介导的Au/PEG解离或酶介导的PEG裂变诱导膜组装/拆卸.
  • 控制和选择性地将客体原细胞吸收到同体微滴中.

主要成果:

  • 在外部刺激 (光) 或内部触发物 (酶) 的反应下,被关闭的原细胞膜成功组装和拆卸.
  • 可以控制和选择性地将客体原细胞吸收到同体内部.
  • 调整客体原细胞表面特性使得原细胞的初始分类成为可能,并证明了可编程的相互作用.

结论:

  • 开发的子原细胞系统为合成原细胞群体中可触发的跨膜吸收提供了一个全新的全水模型.
  • 这项工作突出了人造膜边界间的表面接触相互作用的编程潜力.
  • 潜在的应用包括原细胞网络,存储和传输微系统和微反应器技术.