肝脏亚细胞结构的调节控制了新陈代谢平衡
Güneş Parlakgül1, Ana Paula Arruda1,2, Song Pang3
1Sabri Ülker Center of Metabolic Research and Department of Molecular Metabolism, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Nature
|March 10, 2022
概括
这项研究揭示了肥胖小鼠肝细胞器官结构的动态变化,特别是内质网膜 (ER). 恢复ER架构改善了细胞和全身新陈代谢,突出了它在健康中的作用.
科学领域:
- 细胞生物学
- 代谢学
- 显微镜
背景情况:
- 细胞在有机体内对代谢过程进行细分,从而形成复杂的组织.
- 了解原生组织中的器官结构及其功能影响仍然是一个挑战.
研究的目的:
- 在完整的肝脏组织中高分辨率解析器官的3D结构组织.
- 为了比较瘦小鼠和肥胖小鼠之间的亚细胞结构.
- 研究改变器官结构对新陈代谢的功能影响.
主要方法:
- 增强的聚焦离子束扫描电子显微镜 (FIB-SEM) 用于高分辨率成像 (8nm同位素像素大小).
- 基于深度学习的自动图像细分和大型组织体积的3D重建 (>2.8 × 10^5 μm3).
- 对瘦小鼠和肥胖小鼠的肝组织进行比较分析.
主要成果:
- 肝细胞内有机细胞结构的详细3D重建.
- 在肥胖小鼠中发现了实质性的结构变化,特别是在肝脏内分泌网 (ER).
- 在肥胖小鼠中观察到ER表的明显失调和ER管的占主导地位.
结论:
- 肝脏ER结构高度动态,与新陈代谢状态相结合.
- 对于适应性平衡和组织健康, ER 的结构重组至关重要.
- 实验性恢复细胞下组织影响细胞和系统代谢.
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