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可编程的无细胞转录开关用于抗体检测

Aitor Patino Diaz1, Sara Bracaglia1, Simona Ranallo1

  • 1Department of Chemistry, University of Rome, Tor Vergata, Via della Ricerca Scientifica, Rome 00133, Italy.

Journal of the American Chemical Society
|March 22, 2022
PubMed
概括

我们开发了一种无细胞转录系统, 这种可编程的方法可直接在血清中提供灵敏,快速和经济的抗体检测.

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Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...