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从老鼠多能干细胞获得的功能原生生殖细胞类细胞

Mami Oikawa^1,2, Hisato Kobayashi³, Makoto Sanbo²

¹Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

Science (New York, N.Y.)

|April 7, 2022

概括

科学家从干细胞中产生了功能性老鼠原生生殖细胞类细胞 (PGCLC). 这些细胞在移植时成功地产生了可活的后代,进而促进了体外生殖的研究.

科学领域:

生殖生物学
干细胞研究
发育生物学

背景情况:

从多能干细胞 (PSC) 进行体外生育对生殖医学和动物育种具有前景.
虽然在小鼠中已经确立,但在其他物种中还没有达到诱导原始生殖细胞样细胞 (PGCLC) 的效果.
这项研究的重点在于在老鼠中实现这一里程碑.

研究的目的:

来证明从老鼠PSC中诱导功能性的PGCLC.
研究性腺体细胞在PGCLC成熟和重编程中的作用.
评估大鼠PGCLC产生的生育后代的潜力.

主要方法:

从老鼠PSC中诱导PGCLC,使用浮动类细胞聚合物.
与生殖腺体体细胞共同培养以成熟.
将PGCLC移植到没有生殖线的老鼠的精管中.

主要成果:

从老鼠PSC成功诱导功能性的PGCLC.
证明淋巴体细胞支持PGCLC成熟和表观遗传重编程.
在移植后能够生育出活力后代的功能性精子.

结论:

鼠的PGCLC可以从PSC中功能性诱导.
阴道体细胞对于PGCLC的发育和重编程至关重要.
这种老鼠模型提供了关于体外生育的保存和分离机制的见解,为更广泛的应用铺平了道路.

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