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通过使用Siglec连接体装饰的抗受体抑制免疫反应

Maidul Islam¹, Britni M Arlian¹, Fabian Pfrengle¹

¹Department of Molecular Medicine, and Department of Immunology & Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.

Journal of the American Chemical Society

|May 20, 2022

概括

结合Siglec对抗体可以抑制免疫细胞的激活. 这种方法有效调节不必要的免疫反应,

科学领域:

免疫学
细胞生物学
分子医学

背景情况:

酸结合型免疫球蛋白 (Siglecs) 主要存在于白细胞上,并参与自我识别.
西格莱克通常具有抑制动机,在激活时抑制免疫细胞的信号传递.
针对激活受体的抗体通常会诱导免疫细胞的激活.

研究的目的:

调查是否将Siglec连接剂与向激活受体的抗体结合起来可以抑制免疫细胞的激活.
探索这种策略对调节不必要的免疫反应的潜力.

主要方法:

对针对B细胞受体IgD和IgE/ FcεR1受体复合物的高亲和性Siglec配体的结合.
用抗IgD与CD22合剂抑制B细胞激活的评估.
通过对抗IgE与CD33合剂抑制乳腺细胞脱粒的评估.
在系统性过敏症小鼠模型中对抗IgE- CD33L疗效的体内测试.

主要成果:

对抗IgD抗体的CD22连接显著抑制了B细胞激活.
CD33配体与抗IgE抗体的结合抑制了巨细胞的脱粒化.
在人性化小鼠中,抗IgE- CD33L结合物有效抑制了抗IgE介导的全身过敏反应.
在随后的挑战中,结合剂也防止了过敏反应.

结论:

对抗受体抗体的抑制性Siglec连接可以抑制免疫细胞的激活.
这种新的方法提供了一个调节有害免疫反应的策略.
这些发现表明Siglec-ligand抗体结合物的潜在治疗应用.

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