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相关概念视频

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Anaphase Promoting Complex00:50

Anaphase Promoting Complex

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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相关实验视频

Updated: Sep 7, 2025

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo
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通过Zeb2增强剂内的三重突变消除cDC2的发展

Tian-Tian Liu1, Sunkyung Kim1, Pritesh Desai2

  • 1Department of Pathology and Immunology, Washington University in St Louis, School of Medicine, St Louis, MO, USA.

Nature
|June 22, 2022
PubMed
概括

该研究显示,转录因子NFIL3和C/EBP在Zeb2增强剂中竞争,以控制树突细胞原始细胞的分离. 这种竞争对于确定2型树突细胞 (cDC2) 和它们在T辅助细胞反应中的作用至关重要.

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Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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Last Updated: Sep 7, 2025

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科学领域:

  • 免疫学
  • 发育生物学
  • 转录法规

背景情况:

  • 常见的树突细胞原始体 (CDPs) 的分化路径到cDC1和cDC2系不完全理解.
  • 虽然已知像BATF3这样的转录因子会稳定cDC1的后期承诺,但驱动CDP分歧的初始机制仍然难以捉摸.

研究的目的:

  • 阐明控制常见树突细胞原始体 (CDP) 分离的转录机制.
  • 确定前cDC2电池规格的初始要求.

主要方法:

  • 对NFIL3记者小鼠进行分析,以追踪NFIL3表达动态.
  • 通过CUT&RUN和ChIP-seq识别内源性NFIL3结合部位.
  • 通过CRISPR-Cas9调解,对已识别的调控元素进行了体内突变分析.
  • 在突变小鼠中评估树突细胞发育和T辅助细胞反应.

主要成果:

  • NFIL3 暂时与 Zeb2 增强剂结合,在那里与 C/EBPα 和 C/EBPβ 竞争.
  • 在这些部位,NFIL3充当抑制剂,而C/EBP则充当Zeb2表达的支持者.
  • 破坏NFIL3- C/ EBP结合位导致骨髓原体中的Zeb2表达丧失.
  • 这导致前cDC2规范和成熟cDC2发育的完全失败,损害T助手2反应.

结论:

  • 在Zeb2增强器中,通过NFIL3和C/EBP的竞争机制调节了CDP分离到cDC1和cDC2系.
  • 这一调控轴对于cDC2s的发展及其在调控T助手2免疫反应中的功能至关重要.