相关实验视频
Updated: Aug 11, 2026

11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
概括
确定了两种大鼠大脑蛋白激酶C亚种,它们在炭基终端区域有所不同. 单个基因的替代拼接产生了这些独特的蛋白质激酶C酶.
科学领域:
- 分子生物学分子生物学
- 神经科学是一个神经科学.
- 生物化学 生物化学
背景情况:
- 蛋白激酶C (PKC) 是一种酶家族,对细胞信号传递至关重要.
- 在老鼠大脑中存在多个PKC亚种,这意味着复杂的调节作用.
- 了解PKC亚种的结构和功能多样性对于破译神经通路至关重要.
研究的目的:
- 克隆和表达两种不同亚种的老鼠大脑蛋白激酶C.
- 描述这些PKC亚种的生化和运动性质.
- 调查这些亚种的产生分子基础.
主要方法:
- 补充DNA (cDNA) 克隆和表达在COS 7细胞中.
- 生物化学试验以确定酶活性,以应对激活剂 (Ca2+,脂,二甲糖醇).
- 酶动力学和物理性质的分析.
- 部分基因组结构分析.
主要成果:
- 成功表达了两个PKC亚种 (671和673氨基酸).
- 这两种酶都表现出典型的PKC活性,对标准激活剂作出反应.
- 表达的酶在生化和动力学上与原生老鼠大脑PKC无法区分.
- 序列分析表明,差异仅限于炭基终端区域,这表明了替代拼接.
- 基因组分析证实,这两种亚种都是通过替代拼接从单个基因产生.
结论:
- 鼠脑蛋白激酶C亚种可以从单个基因的替代拼接中产生.
- 这些亚种在它们的炭基终端区域上有微妙的差异,但它们具有共同的基本酶性质.
- 替代拼接提供了一个机制,在大脑中的PKC家族内产生功能多样性.
相关概念视频
What is Gene Expression?
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

