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相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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相关实验视频

Updated: Sep 5, 2025

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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可编程的转录调制与结构化RNA介导的CRISPR-dCas9复合体

Miao He1, Xiang Zhou1, Zhigang Li1

  • 1School of Chemistry and Materials Science, Department of Polymer Science and Engineering, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, Anhui 230026, China.

Journal of the American Chemical Society
|July 6, 2022
PubMed
概括

研究人员开发了一种使用结构化RNA进行可编程基因控制的新型CRISPR-dCas9系统. 这种系统提供了更高的激活效率,并使得针对微RNA的基因调节能够改善细胞控制.

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科学领域:

  • 分子生物学
  • 基因调控
  • 合成生物学

背景情况:

  • 现有的多模块dCas9 (CRISPR相关蛋白9) 系统提供可控的转录操纵.
  • 在CRISPR-dCas9系统中存在内部控制模块的需求,以提高可编程性.
  • 目前像dCas9-VPR这样的系统被广泛使用,但在效率和控制方面可能存在局限性.

研究的目的:

  • 设计一个具有可编程RNA组件的多模块CRISPR-dCas9系统,用于内部控制.
  • 与传统的CRISPR-dCas9激活剂相比,提高基因激活效率.
  • 开发用于内源基因激活和细胞识别的微RNA响应转录调节平台.

主要方法:

  • 开发一个多模块CRISPR-dCas9系统,其中包含结构RNA作为可编程的控制元件.
  • 引入一个微RNA传感器,以创建一个基于dCas9的平台,对内源微RNA做出反应.
  • 在混合细胞群中应用HCT116细胞的选择性鉴定平台.

主要成果:

  • 这种新型结构RNA成分对基于dCas9的基因调节有较强的控制.
  • 与常用的dCas9- VPR系统相比,实现了更高的转录激活效率.
  • 成功生成了微RNA响应平台,可控制内源基因的激活和选择性细胞识别.

结论:

  • 开发的多模块CRISPR-dCas9系统为可编程的基因调节提供了灵活和高效的平台.
  • 整合结构化RNA组件可以提高控制和激活效率.
  • 这种平台在合成生物学,诊断和向基因治疗方面具有潜在的应用.