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废水测序揭示了早期的SARS-CoV-2变种传播

Smruthi Karthikeyan¹, Joshua I Levy², Peter De Hoff^3,4,5

¹Department of Pediatrics, University of California San Diego, La Jolla, CA, USA.

|July 7, 2022

概括

废水基因组监测可以在临床测试之前检测出新的SARS-CoV-2变种. 这种方法通过追踪病毒传播和识别传播来改善公共卫生,即使在资源有限的环境中也是如此.

科学领域:

环境微生物学
病毒学
公共卫生监督

背景情况:

早期检测SARS-CoV-2变种对于有效的公共卫生干预至关重要.
临床测试对血统的流行往往是不可行的,并且可能会有偏见.
污水监测提供了一种不太偏见的方法来追踪区域感染动态.

研究的目的:

开发和部署改进的废水基因组监测方法,以早期检测SARS-CoV-2变种.
克服低质量的序列数据和废水中的相对血统丰度估计的局限性.
将基于废水的基因组监测与295天的临床测序进行比较.

主要方法:

为废水样本开发了增强的病毒度协议.
部署了分解软件, 来解决混合废水样本中的多种病毒菌株.
在大学校园和周边县进行了为期295天的废水和临床测序工作.

主要成果:

新出现的SARS-CoV-2变种在废水中被检测到最早14天.
通过临床基因组监测未发现的病毒传播情况.
已证明能够更准确地从废水中分离多种病毒株.

结论:

废水基因组监测为早期检测SARS-CoV-2变种提供了可扩展的解决方案.
这种方法提高了社区流行率的估计,并确定了神秘的传播.
改善废水监测可以显著帮助公共卫生应对不断变化的病毒威胁.

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