通过闭环结合的功能性RNA的无模板组合
Long-Fei Wu1,2,3,4, Ziwei Liu1, Samuel J Roberts1
1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, United Kingdom.
Journal of the American Chemical Society
|July 26, 2022
概括
研究人员表明,复杂的RNA分子可以在生命的早期形成. 短RNA链自组合成没有模板的功能性核酶,克服了早期RNA复制的挑战.
科学领域:
- 生命研究的起源
- 分子进化
- 核糖核酸生物化学
背景情况:
- 早期的RNA复制可能涉及非酶模板复制.
- 功能性RNA具有复杂的折叠结构,这给复制带来了挑战.
- 补充链对 ribozyme 功能的抑制是一个重要的进化障碍.
研究的目的:
- 将复杂的RNA结构与早期的复制机制相协调.
- 在没有外部模板的情况下研究组装功能性 ribozymes 的途径.
- 解决早期RNA系统中模板介导抑制的挑战.
主要方法:
- 具有突起的短RNA复合体的非酶交叉链结合.
- 将复合体转化为RNA干环结构.
- 用于组装全长 ribozymes 的闭环结合反应.
主要成果:
- 已证明RNA复合体的非酶转化为干环结构.
- 通过闭环结合来展示功能性 ribozymes 的组装.
- 建立了复杂的功能性RNA形成的无模板途径.
结论:
- 在早期进化过程中提出复杂的功能RNA出现的可信途径.
- 表明早期的原细胞基因组可能包含短复制的寡核酸的集合.
- 在早期基于RNA的生命中提供克服复制挑战和模板抑制的机制.
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