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稳定,明亮和长光寿命的染料用于深近红外生物成像

Jin Li^1,2, Yan Dong^1,2, Ruwei Wei^1,2

¹State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Journal of the American Chemical Society

|July 29, 2022

概括

研究人员开发了用于深近红外 (NIR) 生物成像的新替代罗达胺染料. 这些明亮,光稳定和低毒性光体使得体内血管系统的高分辨率成像成为可能.

科学领域:

有机化学有机化学
生物医学成像技术生物医学成像技术
材料科学材料科学材料科学

背景情况:

距离800nm以外的近红外 (NIR) 光体对于深层组织生物医学应用至关重要.
现有的-罗达胺染料仅限于红色光谱区域.
具有理想性质的深NIR光体的合理设计仍然具有挑战性.

研究的目的:

为深度NIR生物成像合成和表征新的替代 bis-benzannulated 罗达胺染料.
评估它们的光物理性质,稳定性,毒性和体内成像能力.

主要方法:

采用五步级联合成方法,在格拉姆尺度上制备了四种替代双化罗达胺染料 (ESi5a-ESi5d).
测量了光物理特性,包括吸收/排放最大值,灭绝系数,光寿命和量子产量.
评估了对光漂白,核友和活性氧物种的稳定性.
在尾部静脉注射ESi5a后,对裸体小鼠血管进行了体内成像.

主要成果:

合成的ESi5a-ESi5d染料在865nm左右表现出强烈的吸收,具有高的灭绝系数 (>10^5cm^-1 M^-1).
刚性结构赋予长光寿命 (>700 ps) 和高量子产量 (0.14).
这些染料表现出优越的光稳定性 (比ICG长2个数量级) 和在生物条件下稳定性,毒性最小.
使用ESi5a.a实现了小鼠血管系统的高信号与背景比成像.

结论:

新型ESi5染料代表了深度NIR光体开发的重大进步.
它们的特殊光物理特性,稳定性和生物相容性使得它们在各种生物成像应用中非常有前途.
这些染料为深度NIR光谱区域的高分辨率体内成像提供了一个新的工具.

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