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PABP/富含素的基因作为一种启动模块,用于模式触发免疫的独立转换

Jinlong Wang¹, Xing Zhang¹, George H Greene¹

¹Howard Hughes Medical Institute, Duke University, Durham, NC 27708, USA; Department of Biology, Duke University, Durham, NC 27708, USA.

|July 30, 2022

概括

植物利用独特的R-动机内部核糖体进入部位 (IRES) 在应激过程中选择性翻译防御mRNA,独立于GCN2/eIF2α通路. 这一过程涉及多A结合蛋白和MPK3/6酸化,将免疫信号与蛋白质合成联系起来.

科学领域:

植物分子生物学
应激反应机制
基因表达的调节

背景情况:

在压力下,真核生物通常通过GCN2/eIF2α信号重新编程翻译.
然而,植物表现出独立于这种途径的应激反应.
在植物中,模式触发免疫 (PTI) 涉及mRNA切割以抑制一般翻译.

研究的目的:

阐明植物PTI过程中选择性防御mRNA翻译的机制.
确定R-动机依赖转换的因素.
建立PTI信号与蛋白质合成调节之间的联系.

主要方法:

在PTI过程中分析mRNA.
识别R-motif作为内部核糖体进入点 (IRES) 的功能.
研究聚A结合蛋白 (PABPs) 和翻译启动因子 (eIFiso4G,eIF4G) 的相互作用.
对基因激活蛋白激酶3和6 (MPK3/6) 酸化效应的研究.

主要成果:

PTI诱导mRNA分离,抑制一般的翻译.
含有R动机的防御mRNA通过IRES活动进行选择性翻译.
对于R-动机翻译,PABP优先使用eIFiso4G而不是eIF4G.
化MPK3/6增强了PABP与R基因的结合,并促进了eIFiso4G介导的翻译.

结论:

植物在PTI期间使用R-motif/PABP/eIFiso4G模块进行选择性防御mRNA翻译.
这种途径将PTI信号与蛋白质合成调节联系起来,绕过GCN2/eIF2α.
PABP/R-motif转化模块可能在真核体中压力转化体重编程中起到保留作用.

关键词:

美国公共交通局在MPK3/MPK6 美国一个R-动机一个RACK 独立于上限的翻译其他国家没有.模式触发的免疫力转换性重编程

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