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Updated: Sep 1, 2025

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
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实时测量可以记录单个细胞的时间转录

Wanze Chen1,2,3, Orane Guillaume-Gentil4, Pernille Yde Rainer1,2

  • 1Laboratory of Systems Biology and Genetics, Institute of Bio-engineering and Global Health Institute, School of Life Sciences, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.

Nature
|August 17, 2022
PubMed
概括

在保持细胞活性的同时,Live-seq可实现单细胞转录组概况. 这种创新方法可以随着时间的推移跟踪细胞行为, 将单细胞RNA测序转化为时间分析方法.

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RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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