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Updated: Sep 1, 2025

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在小鼠和人体组织中可访问色素的空间分析

Yanxiang Deng^1,2, Marek Bartosovic³, Sai Ma⁴

¹Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

|August 17, 2022

概括

空间ATAC-seq绘制了组织中的染色质可访问性,揭示了对于理解细胞发育和疾病至关重要的表观遗传特征. 这种新方法为组织组织和细胞状态提供了基因组规模的洞察力.

科学领域:

表观遗传学
空间生物学
基因组学

背景情况:

细胞功能依赖于本地组织环境,需要先进的空间绘制技术.
虽然空间转录学绘制了基因表达的地图,但细胞和基因组尺度上的空间表观遗传特征缺乏.

研究的目的:

引入一种新的空间分辨的染色体可访问性分析方法.
在组织环境中实现基因组规模的表观遗传映射.

主要方法:

通过结合现场Tn5转换化学和微流体确定性条形编码来开发空间ATAC-seq.
应用下一代测序进行组织剖析.

主要成果:

在小鼠胚胎中划分特定组织区域的表观遗传景观,识别中枢神经系统发育中的基因调节者.
在小鼠和人类大脑中绘制可访问的基因组,揭示复杂的区域区域化.
在桃体组织中的空间特异性免疫细胞组织的分辨率.

结论:

空间ATAC-seq通过提供空间分辨的染色体可访问性概况来推进空间生物学.
这项技术增强了对细胞身份,状态和命运决定的理解,这些决定与发育和疾病中的表观遗传基础有关.

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相关概念视频

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...