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相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Common devices, including car instrument panels, battery chargers, and inexpensive electrical instruments, measure potential difference (voltage), current, or resistance using a d'Arsonval galvanometer. This electromechanical instrument is also known as a moving coil galvanometer.
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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相关实验视频

Updated: May 6, 2026

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
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使用用于双光子显微镜的快速指示器进行持续深层组织电压记录

Zhuohe Liu1, Xiaoyu Lu2, Vincent Villette3

  • 1Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USA.

Cell
|August 19, 2022
PubMed
概括
此摘要是机器生成的。

我们开发了JEDI-2P, 一种新的遗传编码电压指示器, 显著提高了双光子显微镜的性能,

关键词:
美国一个JEDI飞机的视力基因编码的电压指示器高通量选双向电压相关性随机访问显微镜星爆亚马克林细胞双光子光显微镜电压成像

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相关实验视频

Last Updated: May 6, 2026

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科学领域:

  • 神经科学
  • 生物技术
  • 显微镜

背景情况:

  • 基因编码的电压指示器 (GEVIs) 对于特定电池类型的电压动态监测至关重要.
  • 目前的GEVI表现出性能上的局限性,特别是在双光子显微镜下,阻碍了深层组织的神经记录.

研究的目的:

  • 用两光子显微镜优化GEVI以提高性能.
  • 开发一个新的GEVI,JEDI-2P,提高速度,亮度,灵敏度和光稳定性.

主要方法:

  • 开发一个多参数高吞吐量平台来优化GEVI.
  • 应用该平台来识别和描述JEDI-2P.
  • 在Drosophila,小鼠视网膜和清醒的小鼠中对JEDI-2P的体内和体外验证.

主要成果:

  • 与现有的指标相比,JEDI-2P显示出更高的速度,亮度,灵敏度和光稳定性.
  • 在Drosophila内部神经元和小鼠视网膜皮细胞中成功报告了光唤起的反应.
  • 通过共振扫描和ULoVE显微镜在清醒的小鼠中实现单个皮质神经元的长期光学记录.
  • 用JEDI-2P进行的ULoVE记录检测到深度超过400μm的尖峰,并揭示了神经元电压的相关性.

结论:

  • JEDI-2P代表了GEVI在双光子显微镜中的重大进步.
  • 这种指标可以在深度神经回路中进行高分辨率电压成像.
  • 在体内研究神经电路功能的新途径.