植物细胞表面受体复合体的细胞外pH感应
Li Liu1, Wen Song2, Shijia Huang3
1Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong 518055, China; Max-Planck Institute for Plant Breeding Research, Cologne 50829, Germany.
Cell
|August 23, 2022
概括
植物受体复合体感知细胞外pH值,调节根的生长和免疫力. pH的变化改变了受体相互作用,影响了植物的发育和防御反应.
科学领域:
- 植物生物学
- 分子植物科学
- 植物生理学
背景情况:
- 细胞外pH值是植物生物过程的关键调节者.
- 植物感知细胞外pH的机制尚不清楚.
- 了解pH传感对于解读植物生长和免疫调节至关重要.
研究的目的:
- 研究植物如何感知和响应细胞外pH值的变化.
- 阐明受体复合体在pH感应中的作用.
- 了解pH感应对根垂层 (RAM) 生长和免疫的影响.
主要方法:
- 研究了模式触发免疫 (PTI) 对RAM细胞外pH的影响.
- 在不同的pH条件下分析了根干生长因子1 (RGF1) 和它的受体 (RGFRs) 之间的相互作用.
- 在不同的pH值下检查植物诱导 (Peps) 与它们的受体 (PEPRs) 的结合.
- 在RGFR和PEPR之间进行域交换以评估功能的pH依赖性.
主要成果:
- 在RAM中诱导细胞外化,这对于RGF1介导的生长至关重要.
- 细胞外化通过硫胺抑制了依赖酸的RGF1-RGFR相互作用.
- 细胞外化通过Glu/Asp促进了依赖的Pep-PEPR结合,增强了免疫力.
- 域互换改变了RAM生长的pH敏感度,证实了受体域的作用.
结论:
- 植物受体复合体作为细胞外pH传感器.
- 这些复合体的细胞外pH感应可以差异调节植物生长和免疫力.
- 这种机制提供了植物如何整合环境pH线索的发展和防御的见解.
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