循环ADP核糖异构体:生产,化学结构和免疫信号
Mohammad K Manik1,2, Yun Shi3, Sulin Li1,2
1School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
Science (New York, N.Y.)
|September 1, 2022
概括
循环腺二酸盐 (ADP) - 核糖 (cADPR) 异构体是由细菌和植物TIR域产生的. 这些分子,包括3
科学领域:
- 生物化学
- 分子生物学
- 微生物学
背景情况:
- 循环腺二酸盐 (ADP) - 核糖 (cADPR) 异构体是信号分子.
- 细菌和植物Toll/interleukin-1受体 (TIR) 域通过尼古丁胺氨基二核酸 (氧化形式) (NAD+) 水解产生cADPR异构体.
研究的目的:
- 通过TIR域阐明cADPR异构体生成的分子机制.
- 研究cADPR异构体在细菌和植物系统中的生物学作用.
主要方法:
- 对产生2cADPR的TIR域进行结构分析.
- 位点定向的突变发生,以确定循环化的关键残留物.
- 生物化学测试以确定3'cADPR的活性.
主要成果:
- 在ADPR中,v-cADPR (2'cADPR) 和v2-cADPR (3'cADPR) 异构体通过ribose部分之间的O-糖化键形成而形成.
- TIR域结构揭示了导致活跃组件的形状变化.
- 对于cADPR循环形成,保存的基是必不可少的.
- 在Thoeris系统中激活细菌ThsA效应蛋白.
- 当由HopAM1效应器产生时,3'cADPR会抑制植物免疫力.
结论:
- 这项研究揭示了TIR域的cADPR异构体合成的分子基础.
- 3'cADPR 作为细菌中的抗病毒信号分子.
- 3'cADPR作为一种植物免疫抑制剂.
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