使用μMap的辅助严重急性呼吸综合症冠状病毒2宿主进入因子的光化学识别
Saori Suzuki1, Jacob B Geri2, Steve D Knutson2
1Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States.
Journal of the American Chemical Society
|September 1, 2022
概括
严重急性呼吸系统冠状病毒2 (SARS-CoV-2) 使用多个宿主细胞受体进入. 这项研究确定了8种新型受体,它们与ACE2一起增强病毒吸收,有助于理解COVID-19和开发治疗方法.
科学领域:
- 病毒学
- 细胞生物学
- 生物化学
背景情况:
- 严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 是导致COVID-19大流行的原因.
- 血管素转化酶2 (ACE2) 是主要受体,但其低表达表明其他因素也参与了病毒的进入.
- 识别辅助受体对于了解SARS-CoV-2病理生理学和开发对策至关重要.
研究的目的:
- 在人体细胞中快速分析SARS-CoV-2尖端蛋白互动组.
- 确定促进SARS-CoV-2进入的新宿主因素.
- 验证候选受体在病毒吸收中的功能作用.
主要方法:
- 使用光催化近距离标记平台,μMap,用于宿主病毒互动形.
- 使用SARS-CoV-2伪病毒吸收试验系统验证候选受体.
- 测试了武汉和三角洲两种变种的病毒吸收率.
主要成果:
- 确定了与SARS-CoV-2尖端蛋白相互作用的八种新型候选受体.
- 证明ACE2与神经素-2,以素受体A7,溶解载体家族6成员15或髓和淋巴细胞蛋白2的双重表达显著增强病毒吸收.
- 展示了多个宿主因子的协同作用,以有效地进入SARS-CoV-2细胞.
结论:
- SARS-CoV-2 使用多种宿主因子协同进入细胞.
- μMap平台是快速宿主病毒互动组分析的有效工具.
- 这些已识别的受体是新型抗病毒策略的潜在目标.
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