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Updated: May 6, 2026

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分泌人类TNF/cachectin的瘤在小鼠中诱导缓解症

A Oliff, D Defeo-Jones, M Boyer

|August 14, 1987

概括

瘤坏死因子 (TNF) /cachectin在携带瘤的小鼠中引起严重的体重减轻和缓解症. 这一发现表明TNF/cachectin在与癌症相关的衰竭中起着关键作用,影响患者的预后.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

癌症厌食症和减肥显著恶化了患者的结果.
瘤缩因子 (TNF) /cachectin是癌症缩症的疑似原因之一.

研究的目的:

调查TNF/cachectin是否可以诱导瘤动物逐渐减肥.
为研究TNF/cachectin诱导的缓解症建立一个模型.

主要方法:

隔离并将人类TNF/cachectin基因插入表达向量.
感染过的CHO细胞可以产生TNF/卡切分泌细胞系 (CHO/TNF-20).
接种了CHO/TNF-20细胞或对照细胞 (CHO/CMV-Neo) 的裸体小鼠.

主要成果:

与对照组相比,注射CHO/TNF-20细胞的小鼠的生存时间较短.
在肌内CHO/TNF-20瘤的小鼠中,有87%的小鼠患有严重的缓冲症和体重减轻.
对照组的小鼠保持或增加体重.

结论:

从瘤中分泌的TNF/cachectin诱导了小鼠的渐进性衰竭.
TNF/cachectin有助于加速瘤动物的死亡率.
这项研究验证了TNF/cachectin作为癌症缓存症的关键媒介.

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