Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Whole Body Regeneration01:33

Whole Body Regeneration

3.6K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
3.6K
Compounds Essential to Human Function01:25

Compounds Essential to Human Function

7.6K
The human body is composed of cells that are fundamentally made up of several different molecules. These molecules are essential to carry out all physiological processes in the body and are broadly classified into organic and inorganic based on their chemical structures.
Inorganic Compounds Essential to Human Functioning
Inorganic compounds essential to human functioning include water, salts, acids, and bases. These compounds are inorganic, i.e., they do not have a carbon-hydrogen bond. Water...
7.6K
Buffer Systems in the Body01:19

Buffer Systems in the Body

5.5K
Chemical buffers play a critical role in the body's regulation of pH levels. These systems contain one or more compounds that stabilize pH changes by neutralizing strong acids or bases. When pH levels drop, hydrogen ions bind to a weak base; when pH levels rise, hydrogen ions are released. This dynamic process helps maintain pH within a narrow and stable range essential for normal physiological function.
A typical buffer system in bodily fluids includes a weak acid and its corresponding...
5.5K
Cellular Adaptation II: Hypertrophy01:26

Cellular Adaptation II: Hypertrophy

78
Hypertrophy is the increase in the size of individual cells, resulting in the enlargement of a tissue or organ. Unlike hyperplasia, which involves an increase in cell number, hypertrophy is characterized by an increase in cell volume. This process often occurs in response to higher functional demand or hormonal stimulation, leading to the production of more structural proteins and organelles, thereby enhancing the cells' work capacity.There are two primary types of hypertrophy:...
78

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Driver-independent <i>lexAop-tdTomato.nls</i> reporter signal in the adult <i>Drosophila</i> proventriculus.

Fly·2026
Same author

Synergistic integration of μ-SPE and ammoniated adduct-based MRM for sensitive UPLC-MS/MS quantification of PEG1000: a comparative study against in-source CID and application to zebrafish tissue distribution.

Analytical and bioanalytical chemistry·2026
Same author

TIPs: a deep learning-guided proteogenomic framework to expand the landscape of transposable element-derived antigens with immunopeptidomics.

Genome biology·2026
Same author

Unlocking New Hope: Advances in the Application of Mesenchymal Stromal Cells in Female Infertility.

Current stem cell research & therapy·2026
Same author

Geometric-Topology-Driven Membrane Design for Suppressing Polysulfide Crossover in Aqueous Redox Flow Batteries.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Electrical coupling between transplanted cardiomyocytes and host myocardium to prevent arrhythmia.

Disease models & mechanisms·2026

相关实验视频

Updated: May 5, 2026

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

8.8K

在人类直接心脏重编程过程中改善收缩性和线粒体功能

Yawen Tang1, Sajesan Aryal2,3, Xiaoxiao Geng1

  • 1Department of Biomedical Engineering (Y.T., X.G., V.G.F., J.Z., Y.Z.), Heersink School of Medicine, School of Engineering, University of Alabama at Birmingham.

Circulation
|September 14, 2022
PubMed
概括

将TBX20添加到重编程尾酒中显著改善了纤维细胞的直接心脏重编程成为功能性心肌细胞. 这提高了心脏功能和线粒体呼吸,为心肌修复提供了有前途的策略.

关键词:
细胞重编程纤维细胞一个心肌细胞,心脏进行再生转录因子

更多相关视频

Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

1.3K
Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes
08:47

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes

Published on: December 16, 2022

2.5K

相关实验视频

Last Updated: May 5, 2026

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

8.8K
Assessing Cardiac Reprogramming using High Content Imaging Analysis
06:02

Assessing Cardiac Reprogramming using High Content Imaging Analysis

Published on: October 26, 2020

1.3K
Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes
08:47

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes

Published on: December 16, 2022

2.5K

科学领域:

  • 心血管生物学
  • 干细胞生物学
  • 分子心脏病学

背景情况:

  • 直接心脏重编程旨在通过将纤维细胞转化为心肌细胞来再生心肌.
  • 目前使用标准尾酒的方法产生不足的人体诱导心肌细胞.
  • 控制高效心脏重编程的分子机制尚不清楚.

研究的目的:

  • 确定促进人体纤维细胞直接转化为功能性心肌细胞的新型因素.
  • 阐明TBX20在心脏细胞命运转换和心肌细胞成熟中的作用.

主要方法:

  • 人体诱导心肌细胞与本源心肌细胞之间的转录组比较.
  • 将TBX20添加到MGT133重编程尾酒 (MEF2C,GATA4,TBX5,miR-133) 中.
  • 包括转录组学,染色体占用和表观组学在内的综合分析.

主要成果:

  • 确定TBX20是标准MGT133尾酒不激活的关键基因.
  • MGT+TBX20混合剂显著提高了心脏重编程效率和心肌细胞功能.
  • 增强的人类诱导心肌细胞表现出更好的跳动,处理,线粒体呼吸和收缩能力.

结论:

  • TBX20与MGT因子协同作用,激活心脏增强剂,促进有效的细胞命运转化.
  • 通过TBX20增强的重编程产生具有卓越功能特征的人体心肌细胞,包括收缩性和线粒体活性.
  • 这种策略有可能开发出更好的基于细胞的心脏病治疗方法.