葡萄糖驱动的TOR-FIE-PRC2信号控制装置的发展
Ruiqiang Ye1,2, Meiyue Wang3,4,5, Hao Du6,7
1Department of Molecular Biology and Centre for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, USA. ye@molbio.mgh.harvard.edu.
Nature
|September 14, 2022
概括
在植物中,由葡萄糖激活的Rapamycin (TOR) 酶通过调节基因组甲基化来控制细胞命运和发育. 这种营养信号传递途径会影响植物的器官生成和向开花的过渡.
科学领域:
- 植物生物学
- 表观遗传学
- 分子信号
背景情况:
- 营养和能量对于植物的发展至关重要.
- 拉帕米辛 (TOR) 激酶的目标集成营养信号以控制生长.
- 在发展过渡和差异化中TOR的作用尚不完全理解.
研究的目的:
- 研究葡萄糖激活的TOR激酶如何影响Arabidopsis thaliana的表观遗传修饰和发育程序.
- 识别和描述参与调节细胞命运和发育的TOR目标.
主要方法:
- 在K27 (H3K27me3) 中对基因组H3三甲基化的全基因组分析.
- 作为TOR目标的FIE的识别.
- 对FIE的酸化位点突变分析.
- 转录组重编程分析
- 对葡萄糖-TOR-FIE-Polycomb抑制复合体2 (PRC2) 信号通路的研究.
主要成果:
- 葡萄糖激活的TOR激酶控制了Arabidopsis的全基因组H3K27me3水平.
- 独立于施肥的精子末端是TOR的直接目标,其酸化由TOR驱动核转移.
- 破坏FIE酸化会废除H3K27me3,改变转录组,并损害器官生成.
- 葡萄糖-TOR-FIE-PRC2通路调节了化诱导的花过渡.
结论:
- 葡萄糖-TOR-FIE-PRC2信号轴作为营养检查点.
- 这种途径在表观遗传上使控制干细胞命运和器官模式的基因沉默.
- 营养信号直接对表观基因组进行重新编程,从而影响植物的发育转变.
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