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光生物传感器用于测量活细胞中的Ras活性

Ryan Weeks1,2, Xin Zhou1, Tina L Yuan3

  • 1Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.

Journal of the American Chemical Society
|September 19, 2022
PubMed
概括

科学家开发了RasAR, 这是一种测量活细胞Ras蛋白活性的新工具. 这位记者有助于了解Ras在癌症中的调节, 并评估新的癌症药物的有效性.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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