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Updated: Aug 28, 2025

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通过微生物糖化物酶激活植物受体样蛋白

Yue Sun¹, Yan Wang^2,3, Xiaoxiao Zhang¹

¹Tsinghua-Peking Center for Life Sciences, Beijing Advanced Innovation Center for Structural Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.

|September 21, 2022

概括

植物免疫受体,受体样蛋白 (RLPs),识别病原体的信号. RXEG1 RLP结构揭示了它如何结合XEG1,通过共同受体BAK1抑制病原体并触发植物免疫力.

科学领域:

植物免疫力
分子植物病原体相互作用
结构生物学

背景情况:

植物利用细胞表面模式识别受体 (PRR) 来检测危险信号并启动免疫反应.
具有丰富白重复 (LRR) 的受体样蛋白 (RLPs) 是植物免疫中的关键PRR.
对于LRR-RLPs的相关体识别和激活的精确机制尚未完全理解.

研究的目的:

阐明LRR-RLP中的配体识别和激活的结构基础.
研究RXEG1 RLP与Phytophthora sojae效应器XEG1之间的相互作用.
了解RXEG1-XEG1相互作用如何触发下游免疫信号通路.

主要方法:

使用X射线结晶学来确定Nicotiana benthamiana中的LRR-RLP RXEG1的结构,无论是单独的还是与XEG1和共同受体BAK1的复合.
使用生物化学测定来确认RXEG1对XEG1酶活性的抑制作用.
进行结构比较以分析由连接体结合引起的形状变化.

主要成果:

RXEG1的晶体结构显示,XEG1的特定识别是由其氨基终端和碳基终端循环区域 (RXEG1 ((ID)) 介导的.
这些环结合在XEG1的活性位点槽内,有效抑制其酶活性并抑制Phytophthora感染.
结合XEG1诱导RXEG1的结构变化,通过保留的LRR域促进其与共受体BAK1的结合,从而启动免疫信号.

结论:

已发现LRR-RLP与BAK1共受体之间的联体诱导异构化.
LRR-RLP RXEG1具有双重功能:直接抑制病原体效应体和激活植物免疫信号.
这项研究为植物如何感知病原体衍生的分子并建立有效的免疫防御提供了关键的结构见解.

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