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相关概念视频

Coordination of Gene Expression Processes in Bacteria01:29

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The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
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The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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相关实验视频

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在细微细节上可视化细菌细胞内的转化动态

Liang Xue1,2, Swantje Lenz3, Maria Zimmermann-Kogadeeva1

  • 1Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.

Nature
|September 28, 2022
PubMed
概括

这项研究使用冷电子断层扫描实时可视化细菌蛋白质合成 (翻译). 它揭示了核糖体结构和动力学,包括核糖体蛋白质L9如何协调多元体以实现准确的翻译.

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科学领域:

  • 结构生物学
  • 分子生物学
  • 微生物学

背景情况:

  • 翻译是一种基本的细胞过程, 对于蛋白质合成至关重要,
  • 在体内了解核糖体动力学对于解读细胞机制和开发向疗法至关重要.

研究的目的:

  • 以高分辨率可视化细菌Mycoplasma pneumoniae中的翻译结构动态.
  • 激活翻译延长并研究本地细胞环境中的多元体形成和协调.

主要方法:

  • 使用冷电子断层扫描和亚断层扫描分析进行细胞内结构可视化.
  • 获得高分辨率的细胞内转化核糖体平均图,并建立原子模型.
  • 分类了不同的核糖体状态,并绘制了细胞内多元体组织.

主要成果:

  • 解决了13个不同的核糖体状态,重复了已知的体外状态并反映了活跃的转化中间体.
  • 在原生细胞内动画翻译延长,展示抗生素对细胞翻译的影响.
  • 通过核糖体蛋白L9调解的多元体协作的局部协调机制.

结论:

  • 这项研究证明了可视化分子过程的可行性,
  • 这些发现提供了对翻译忠实性,多体组织以及抗生素对细菌翻译的影响的见解.