Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gene Conversion02:08

Gene Conversion

9.9K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.9K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

10.0K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
10.0K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
Exon Recombination02:32

Exon Recombination

3.7K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.7K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.1K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Management of the Axilla for Early-Stage Breast Cancer: A State-Of-The-Art Review.

ANZ journal of surgery·2026
Same author

Emergent domain segregation in self-interacting polymers explains chromosome 3D conformations in single human cells.

Physical review. E·2026
Same author

PSMA PET/CT staging in intermediate-risk prostate cancer: Toward risk-adapted implementation.

Seminars in oncology·2026
Same author

ZNF512B safeguards genome integrity at regulatory regions to repress the SASP and inflammation.

Cell stem cell·2026
Same author

Overcoming absolute dysphagia in a thirty-year-old patient with advanced anaplastic lymphoma kinase-positive non-small cell lung cancer: a case report.

Frontiers in oncology·2026
Same author

Physics-Based Modeling of Sparse Single-Cell Hi-C Uncovers Structural and Epigenetic Variability.

International journal of molecular sciences·2026

相关实验视频

Updated: Aug 27, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

10.4K

抑制和3D重组解决了进化重新排列基因组中的监管冲突

Alessa R Ringel1, Quentin Szabo2, Andrea M Chiariello3

  • 1Max Planck Institute for Molecular Genetics, Berlin, Germany; Institute for Medical and Human Genetics, Charité Universitätsmedizin Berlin, Berlin, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin, Berlin, Germany.

Cell
|September 30, 2022
PubMed
概括

新基因可以融入现有的调节区域,而不会破坏它们. 在特定的背景下,DNA甲基化防止干扰,允许独立的基因表达和进化灵活性.

关键词:
三维基因组组织在CTCFDNA 甲基化一致性发展基因调节增强剂-促进剂特异性一个进化面膜相关的领域循环挤出在拓上关联域

更多相关视频

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

12.7K
A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.5K

相关实验视频

Last Updated: Aug 27, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
11:25

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

10.4K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

12.7K
A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

5.5K

科学领域:

  • 基因组学
  • 发育生物学
  • 进化生物学

背景情况:

  • 调控环境对于基因表达在发育过程中至关重要.
  • 在新基因的结合过程中保持调节完整性是不太了解的.
  • 拓相关域 (TAD) 组织基因组并影响基因调节.

研究的目的:

  • 研究一种哺乳动物特异性基因 (Zfp42) 如何融入古老的TAD而不会影响现有的基因 (Fat1) 的表达.
  • 阐明在进化过程中在共享的TAD中保持独立基因调节的机制.
  • 确定TAD内部的调节复杂性是否是一种常见的进化现象.

主要方法:

  • 在胚胎干细胞 (ESC) 和胚胎肢体中研究了Zfp42和Fat1的基因表达和调控机制.
  • 分析了色素活性,CTCF/凝聚素结合,增强剂活性和DNA甲基化模式.
  • 检查了TAD分区和核封面的附着.
  • 在TAD中进行基因分布的全基因组分析.

主要成果:

  • 通过TAD分区在ESC中物理分离Zfp42和Fat1,不同的增强剂驱动独立表达.
  • 染色体活动,而不是CTCF/凝聚素,驱动ESC的这种分离.
  • 在胚胎肢体中,Zfp42在Fat1的TAD中不活跃,对Fat1增强剂没有反应.
  • 而不是增强剂不兼容性或核附着性, 解释了Zfp42的无反应性.
  • 大多数TAD全基因组包含多个独立表达的基因.

结论:

  • 多种机制,包括染色体活性和上下文依赖的DNA甲基化,促进独立调节的基因在现有的基因位点内集成.
  • 在TAD中的调节复杂性是脊椎动物进化的共同特征.
  • 这项研究提供了基因组如何适应新基因,同时保持调控完整性的见解.