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Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Updated: Jun 15, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

脂质扩展显微镜

Brittany M White^1,2, Pratik Kumar³, Amanda N Conwell^1,2

¹Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States.

Journal of the American Chemical Society

|October 3, 2022

概括

此摘要是机器生成的。

研究人员开发了脂质扩展显微镜 (LExM) 用于细胞膜的超高分辨率成像. 这种方法精确地可视化脂和有机细胞膜,克服光显微镜的局限性.

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Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

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Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

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Last Updated: Jun 15, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

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Published on: January 31, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

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Published on: October 6, 2023

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

科学领域:

细胞生物学
显微镜
生物化学

背景情况:

光显微镜受到衍射的限制,阻碍了像脂质双层这样的纳米级细胞结构的可视化.
现有的超分辨率技术往往需要复杂的标签或专门的设备.

研究的目的:

开发一种新的超分辨率成像方法,用于细胞膜内的脂.
为了克服膜结构的精确可视化.

主要方法:

开发了脂质扩展显微镜 (LExM),一种完全小分子的方法.
在水凝网络中化学定生物正角标记的脂.
扩展显微镜的应用原理以提高分辨率.

主要成果:

在细胞膜中实现了代谢标记脂的超高分辨率成像.
精确的可视化器官膜,包括核侵蚀.
已证明与标准的共焦显微镜兼容.

结论:

通过LExM,可以对细胞膜和脂进行高精度超分辨率成像.
该方法克服了可视化纳米尺度膜结构的衍射限制.
对于研究各种生理环境而言,LExM具有广泛的适用性.