结合细胞温度测试显示,Salmonella typhimurium通过诱导类似于Pyroptosis的表型来温暖巨细胞
Leyang Wu1,2, Feng Chen3, Xiaoyao Chang1
1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing210023, China.
Journal of the American Chemical Society
|October 13, 2022
概括
沙门氏甲状病毒VNP20009通过涉及线粒体的过程 - - 热死,增加了巨细胞的温度. 这种细菌菌株也会导致局部瘤变暖,促进M1巨细胞的两极分化.
科学领域:
- 免疫学
- 微生物学
- 生物物理
背景情况:
- 沙门氏型VNP20009表现出抗瘤特性,并招募巨细胞.
- 在巨细胞中,VNP20009引起的特异性细胞变化,如温度变化,尚不清楚.
研究的目的:
- 研究VNP20009对巨细胞温度的影响.
- 阐明VNP20009引起的细胞温度变化的机制及其与热和瘤微环境的关系.
主要方法:
- 实时无线多细胞测温系统用于测量巨细胞温度.
- 使用VNP20009, ΔsipD-VNP20009菌株和Gsdmd-/ - 大细胞来研究热.
- 单细胞温度计和线粒体功能的评估.
主要成果:
- VNP20009显著增加了0. 2°C的巨细胞温度.
- 由VNP20009和尼日里辛诱导的巨炎与细胞升温直接相关.
- 线粒体在VNP20009诱导的细胞升温中发挥着关键作用,而瘤升温在巨细胞清除时减少,与M1偏振相关.
结论:
- 巨炎是VNP20009引起的细胞升温的关键媒介.
- 线粒体在热过程中对生物热信号传导至关重要.
- VNP20009诱导的瘤变暖影响了巨细胞的两极分化,为细菌与宿主相互作用和生物热信号提供了洞察力.
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