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蝶翅膀图案的深度Cis调节同质性地面图

Anyi Mazo-Vargas^1,2, Anna M Langmüller³, Alexis Wilder²

¹Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, USA.

Science (New York, N.Y.)

|October 20, 2022

概括

蝶的翅膀模式从古老的遗传蓝图迅速演变. 科学家们发现, 保护的调节元素, 而不是快速的变化, 解释了蝶颜色模式的深层同质性和多样性.

科学领域:

进化发育生物学
基因组学
昆虫学

背景情况:

蝶的翅膀模式表现出快速的进化叠加在一个保存的发展计划.
这些模式的深层同源性和适应性变化的基因机制尚不清楚.
了解Cis监管的演变是解读模式多样化的关键.

研究的目的:

研究蝶中WntA基因的 cis调节演变.
确定基因调节架构如何适应深层同质性和快速适应性变化.
确定控制蝶颜色模式的保存和分离的cis-regulatory元素.

主要方法:

在五种蝶物种中比较ATAC-seq (通过测序检测可转移酶的染色质).
在体内删除46个已识别的cis-regulatory元素.
调节元件的功能和保存的分析.

主要成果:

在大多数物种中观察到对WntA的基平面决定的深度同质性,在君主蝶中则例外.
非编码删除显示了广泛的正面和负面调节效应,通常影响基因表达的多个方面.
证据表明,祖先的多功能非编码元素对于复杂的特征系统的演变至关重要.

结论:

深度保存,多功能非编码元素,而不是快速增强器周转,可能是多样化的蝶翅膀模式的基础.
WntA的监管架构为保存的发展计划如何产生快速的表型多样化提供了一个模型.
这项研究挑战了在快速演变的特征中预测监管元素的快速转换的模型.

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