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植物中TIR1/AFB辅酶受体的腺酸环酶活性
Linlin Qi1, Mateusz Kwiatkowski2, Huihuang Chen1
1Institute of Science and Technology Austria (ISTA), Klosterneuburg, Austria.
Nature
|October 26, 2022
概括
植物辅酶受体,TIR1/AUXIN-SIGNALING F-BOX (AFB),具有腺酸环酶 (AC) 的活性. 这种cAMP的产生对辅酶信号,植物发育和转录调节至关重要.
科学领域:
- 植物生物学
- 分子生物学
- 生物化学
背景情况:
- 素是通过规范信号通路调节植物发育的关键植物激素.
- 运输抑制剂反应1 (TIR1) / 奥信号F-BOX (AFB) 蛋白质是奥素受体和Ubiquitin Ligase复合体的F-box子单元.
- 这些受体通过向Aux/IAA抑制剂进行降解来调解auxin反应.
研究的目的:
- 研究TIR1/AFB辅酶受体的新功能.
- 在辅酶信号通路中识别新的分子机制.
- 探索循环AMP作为植物中的第二信使的作用.
主要方法:
- 在TIR1/AFB受体中检测腺酸环酶 (AC) 活性的生物化学测试.
- 在TIR1 C终端区域内的AC动机的位点定向突变.
- 对突变植物进行表型分析,以评估引力和根生长.
- 对辅酶诱导的转录调节的分析.
主要成果:
- 在陆地植物中,TIR1/AFB受体表现出腺酸环酶 (AC) 活性.
- 辅酶和辅酶/IAA蛋白通过TIR1/AFB受体刺激cAMP的产生.
- 消除AC活性的突变使得TIR1在调解重力化,根生长抑制和转录调节方面无效.
- 这标识了F盒和AC图案的双重功能接收盒.
结论:
- TIR1/AFB受体具有以前未知的腺酸环酶 (AC) 活性,对于正规的辅酶信号来说至关重要.
- 这种AC活动对于调解关键的植物发育过程至关重要,比如重力培养和根生长.
- 这些发现揭示了cAMP作为植物信号传递中的新型第二信使,突出了具有双重F盒和AC功能的独特植物激素受体.
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