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蓝光损伤蛋白质的化学蛋白质识别

Kohei Toh¹, Kosuke Nishio¹, Reiko Nakagawa²

¹Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.

Journal of the American Chemical Society

|October 28, 2022

概括

蓝光暴露会导致蛋白质损伤,导致细胞功能障碍和衰老. 这项研究确定了蓝光氧化细胞表面蛋白质,

科学领域:

生物化学
细胞生物学
蛋白质组学

背景情况:

可见光,尤其是蓝光,会产生反应性氧物种 (ROS),造成细胞损伤.
生物分子的光氧化与衰老和疾病有关,

研究的目的:

在活哺乳动物细胞中绘制蓝光诱导的蛋白质损伤的化学蛋白质学方法.
识别受蓝光影响的特定蛋白质和细胞通路.

主要方法:

使用核友化学探针的化学蛋白质方法.
分析基因本体丰富,以识别敏感的蛋白质集.
研究蓝光诱导的蛋白质损伤对细胞粘附和增殖的功能影响.

主要成果:

已确定细胞表面蛋白质,特别是整合蛋白 (ITG),对蓝光诱导的氧化非常敏感.
证明蓝光氧化整合蛋白β1 (ITGB1) 的功能受损.
显示光损伤的整合素有助于蓝光引起的细胞功能障碍.

结论:

开发了一种用于绘制蓝光损伤活细胞中的蛋白质的新方法.
突出了细胞表面受体的脆弱性,
这表明整合素光损伤在蓝光诱导的细胞病理中起作用.

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