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Updated: Aug 22, 2025

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research

Author Spotlight: Innovating Thiol Quantification and Biomarker Detection for Oxidative Stress Research

Published on: June 28, 2024

饮食中的抗氧化剂ergothioneine的微生物载体

Daniel G Dumitrescu¹, Elizabeth M Gordon², Yekaterina Kovalyova¹

¹Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA; Department of Chemistry, Yale University, New Haven, CT 06520, USA; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA.

|November 8, 2022

概括

使用EgtUV转运体从宿主中吸收抗氧化剂埃尔戈氨酸 (EGT). 这种饮食EGT有助于微生物殖民,并影响肠道健康,揭示了一种新的氧化还原调节机制.

科学领域:

微生物学
生物化学
人类健康

背景情况:

低分子量 (LMW) 硫醇是细胞氧化还原平衡的关键抗氧化剂.
许多微生物,包括Helicobacter pylori, 缺乏合成这些必需的醇的途径.
在人体组织中含有丰富的食性埃尔戈氨酸 (EGT),与疾病风险减轻有关.

研究的目的:

为了研究Helicobacter pylori如何维持氧化还原稳定,尽管缺乏LMW-thiol生物合成.
确定埃尔戈氨酸被H. pylori获得的来源和机制.
探索微生物在人类肠道中的ergothioneine代谢的更广泛的影响.

主要方法:

以反应为导向的代谢学来识别H. pylori中的LMW硫醇.
EgtUV传送器的遗传和生化特征.
人体便微生物群的体内定居测试和分析.

主要成果:

埃尔戈氨酸 (EGT) 被确定为H. pylori利用的一种不寻常的LMW硫醇.
杆菌通过选择性ATP结合盒传输体EgtUV从宿主环境中获取EGT.
EgtUV为H.pylori的殖民提供了竞争优势,并且在肠道微生物中保存.
人体肠道细菌代谢EGT,可能有助于三甲基胺N-氧化物的产生.

结论:

杆菌采用宿主衍生的抗氧化策略,通过EGT载体获得EGT.
饮食中EGT的跨国竞争是肠道微生物生态和人类健康的一个重要因素.
这项研究揭示了一种涉及宿主-微生物相互作用的新型微生物氧化还原调节机制.

关键词:

一个ABC运输器甲状腺细菌埃尔戈西奥内因主体微生物低分子量硫醇代谢过程微生物组氧化应激氧化还原调节

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