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内微生物群对癌症空间和细胞异质性的影响

Jorge Luis Galeano Niño¹, Hanrui Wu¹, Kaitlyn D LaCourse¹

¹Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.

|November 17, 2022

概括

瘤微生物群不是随机分布的,而是组织在口腔和结直肠癌中的特定位置. 这些以抑制免疫力和改变细胞功能为特征的,促进癌症的进展.

科学领域:

癌症学
微生物学
基因组学

背景情况:

瘤相关的微生物群是瘤微环境的关键组成部分.
以前的研究依赖于大量组织分析,掩盖了微生物群的空间分布和局部影响.

研究的目的:

通过先进的空间分析和单细胞测序,揭示瘤内的空间,细胞和分子宿主微生物相互作用.
了解内微生物群的有组织分布和功能影响.

主要方法:

适应10xVisium空间转录组,用于在现场微生物群体的识别和定位.
使用GeoMx数字空间分析来分析细菌的特征.
开发并应用INVADEseq (入侵-粘附定向表达序列) 用于单细胞宿主-微生物相互作用分析.

主要成果:

在较少血管化的,免疫抑制的微小细胞中发现了具有较低Ki-67水平的恶性细胞.
鉴定了与细胞相关的细菌和相互作用的宿主细胞,揭示了炎症,转移,休眠和DNA修复的改变途径.
证明受细菌感染的癌细胞单独入侵并招募骨髓细胞,促进瘤的进展.

结论:

内微生物分布在特定的微型中高度组织.
这些富含微生物的微生物具有免疫和上皮细胞功能, 积极促进癌症的进展.

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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...