相关实验视频

Updated: Aug 20, 2025

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes

Published on: August 28, 2016

与心肌细胞分化相关的代谢变化使成年哺乳动物的心脏再生

Yuan-Yuan Cheng¹, Zachery Gregorich², Ray P Prajnamitra¹

¹Institute of Biomedical Sciences (Y.-Y.C., R.P.P., T.-Y.M., Y.-H.H., Y.-C.L., S.-C.R., J.-H.L., P.-J.L., Y.-T.Y., P.C.H.H.), Academia Sinica, Taipei, Taiwan.

|November 24, 2022

概括

成人心肌细胞 (CMs) 可以被重新编程以脱差和增殖,促进心脏再生. 这一过程与HMGCS2调节的生成有关,这对心脏受伤后的修复至关重要.

科学领域:

心血管生物学
细胞重编程
代谢调节

背景情况:

成年哺乳动物心肌细胞 (CMs) 在受伤后具有有限的再生能力.
某些动物物种通过CM脱差和增殖表现出心肌再生.

研究的目的:

在体内研究心肌细胞脱差和增殖的机制.
探索生成在心脏再生中的作用.

主要方法:

产生可诱导,心肌细胞特异的Yamanaka因子表达的小鼠用于体内重编程.
使用微阵列分析识别关键分子通路.
使用腺相关病毒 (AAV) 介导的 HMGCS2 过度表达的基因传递.
研究了HMGCS2淘汰小鼠,并评估了心肌梗塞后的心脏功能.

主要成果:

诱导的CM表现出Yamanaka因子表达后的脱差和增加的扩散.
基因生成,特别是HMGCS2上调,被认为是这一过程的核心.
HMGCS2过度表达重复的CM脱差和扩散.
在HMGCS2淘汰的小鼠中显示出心脏功能受损和受伤反应.
外源性HMGCS2的使用可恢复心脏功能.

结论:

HMGCS2诱导的生成对于调节CM损伤的代谢反应至关重要.
这种代谢途径促进心肌细胞脱差和增殖,推动心脏再生.
向代是一种潜在的心脏修复治疗策略.

关键词:

在HMGCS2中细胞脱差细胞增殖细胞重编程基因生成

更多相关视频

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Published on: March 22, 2017

相关概念视频

Forced Transdifferentiation

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...

Myocarditis I: Introduction

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...