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循环核酸激活CRISPR蛋白酶的抗病毒信号

Christophe Rouillon^1,2, Niels Schneberger³, Haotian Chi⁴

¹Institute of Structural Biology, University of Bonn, Bonn, Germany. back2crispr@gmail.com.

|November 24, 2022

概括

与CRISPR相关的Lon蛋白酶 (CalpL) 裂解CalpT释放CalpS,将外来核酸检测与 prokaryotes 中的转录调节联系起来. 这揭示了CRISPR系统与抗菌体信号之间的联系.

科学领域:

微生物学
分子生物学
结构生物学

背景情况:

包括DNA向Cas9和RNA向III类型在内的CRISPR防御系统在原生生物中很常见.
在检测异性RNA时,III型CRISPR系统通过循环基酸盐合成启动抗病毒反应.
预计与III型系统相关的蛋白质,包括与CRISPR相关的Lon蛋白酶 (CalpL),会结合循环基酸盐.

研究的目的:

阐明与CRISPR相关的Lon蛋白酶 (CalpL) 的结构和功能.
了解CalpL与CRISPR相关复合体中的其他蛋白质相互作用的机制.
建立由CRISPR介导的外来核酸识别与下游监管事件之间的联系.

主要方法:

对 CalpL 蛋白的结构分析.
生物化学测试以确定 CalpL 在三方复合体中的功能.
操作子分析以确定相互作用蛋白 (CalpT和CalpS).

主要成果:

与 CalpT 和 CalpS 形成一个稳定的三元复合体.
激活的 CalpL 寡合并分裂 CalpT,这是 MazF 的同类物质.
CalpT的裂变释放了细胞外功能西格玛因子CalpS.
CalpL 具有 SAVED 域,可以结合循环四基酸盐 (cA4).

结论:

这项研究表明,外来核酸的CRISPR检测与转录调节之间存在直接联系.
这些发现揭示了CRISPR效应器与SAVED域和基于循环寡核酸的抗菌信号系统之间的联系.
CalpL的功能突显出一种新型的抗病毒防御和基因调节机制.

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