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Updated: Aug 19, 2025

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
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人类信号酶复合体作为膜蛋白质的质量控制酶

Andrea Zanotti1, João P L Coelho2, Dinah Kaylani2

  • 1Center for Molecular Biology of Heidelberg University (ZMBH), 69120 Heidelberg, Germany.

Science (New York, N.Y.)
|December 1, 2022
PubMed
概括

人类信号酶复合体 (SPC) 作为膜蛋白质的质量控制因子. 它将错误折叠的蛋白质分离到神秘的部位, 针对它们进行降解以维持细胞平衡.

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
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