相关实验视频
Updated: Aug 17, 2025

10:00
RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
Published on: March 4, 2020
11.1K
一个特定于男性生殖细胞的核糖体控制男性生育能力
Huiling Li1, Yangao Huo2,3, Xi He1
1State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.
Nature
|December 14, 2022
概括
研究人员发现一种特殊的精子核糖体, ribosomeST, 对于男性生育至关重要. 这种独特的核糖体确保了精子形成过程中的适当蛋白质折叠,突出显示了哺乳动物的新型组织特异性转化调节.
科学领域:
- 分子生物学
- 细胞生物学
- 生殖生物学
背景情况:
- 核糖体表现出异质性,影响蛋白质合成的调节.
- 男性生殖细胞的发育依赖于精子形成过程中的复杂转化控制.
研究的目的:
- 调查特定的核糖体类型是否负责精子形成过程中的翻译.
- 描述一种新的精子特定核糖体的结构和功能.
主要方法:
- 来自小鼠脏和丸的核糖体的单颗粒冷电子显微镜.
- 在小鼠中的精子特定核糖体的遗传删除.
- 蛋白质折叠调节的分析.
主要成果:
- 用男性生殖细胞特异性RPL39L组装的RibosomeST的鉴定.
- 在小鼠中删除RibosomeST导致精子形成缺陷并降低生育能力.
- 与RibosomeCore相比,RibosomeST具有一个独特的新生聚出口道.
- 核糖体ST通过共译来调节精子形成的基本蛋白质的折叠.
- 核糖体不能取代 RibosomeST 的专业功能.
结论:
- 一种特定于精子的核糖体,RibosomeST,在男性生育中起着至关重要的作用.
- 核糖体ST的独特结构可以调节精子生成过程中的特定蛋白质折叠.
- 这一发现扩大了对核糖体多样性和组织特异性转化控制的理解.
相关概念视频
The Y Chromosome Determines Maleness
6.7K
The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
6.7K
Infertility in Males
314
Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...
314
Spermatogenesis
102.9K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.9K
The Ratio of X Chromosome to Autosomes
8.7K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
8.7K
X and Y Chromosomes
26.5K
Among mammals, the gender of an organism is determined by the sex chromosomes. Humans have two sex chromosomes, X and Y. Every human diploid cell has 22 pairs of autosomes and one pair of sex chromosomes. A human female has two X chromosomes, while a male has one X chromosome and one Y chromosome.
The germline cells such as egg and sperm cells carry only half the number of chromosomes, i.e., 22 autosomes and one sex chromosome. All eggs have an X chromosome, while sperm cells can carry an X or...
The germline cells such as egg and sperm cells carry only half the number of chromosomes, i.e., 22 autosomes and one sex chromosome. All eggs have an X chromosome, while sperm cells can carry an X or...
26.5K
Sex-linked Disorders
102.6K
Like autosomes, sex chromosomes contain a variety of genes necessary for normal body function. When a mutation in one of these genes results in biological deficits, the disorder is considered sex-linked.
102.6K

