超声触发的现场光子发射用于非侵入性光遗传学
Wenliang Wang1, Xiang Wu2,3, Kai Wing Kevin Tang1
1Biomedical Engineering Cockrell School of Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
Journal of the American Chemical Society
|January 6, 2023
概括
研究人员开发了用于聚焦超声触发光辐射的新型脂质体纳米粒子,从而实现对神经元的非侵入性光遗传控制. 这一突破推动了脑电脑接口和神经科学研究.
科学领域:
- 神经科学
- 生物技术
- 医学成像
背景情况:
- 视觉遗传学提供精确的神经电路控制, 但在向大脑提供非侵入性光线方面面临挑战.
- 聚焦超声波 (FUS) 可以通过机械发光产生光,但光遗传学缺乏生物相容的液相系统.
研究的目的:
- 开发一种生物相容的液相机械发光系统,用于使用聚焦超声波的非侵入性时空光遗传学.
- 制造能够通过FUS触发光子发射的神经激活的脂质体纳米粒子.
主要方法:
- 为FUS触发的机械发光合成了脂质体纳米粒子 (Lipo@IR780/L012).
- 在体外实验中评估了FUS下的光发射和素表达细胞激活.
- 在体内研究评估了动物模型中的非侵入性神经激活和运动控制.
主要成果:
- 在FUS照射下,脂质体纳米颗粒显示出同步且稳定的蓝光发射.
- 在体外,FUS成功触发光发和激活素表达细胞.
- 在体内,非侵入性光遗传刺激运动皮质神经元诱导了可逆的肢体运动.
结论:
- 脂质体纳米颗粒为FUS触发的机制发光提供了一个新平台,使非侵入性光遗传学成为可能.
- 这项技术为精确的神经电路调制提供了有前途的解决方案,具有高时间分辨率和生物相容性.
- 开发的系统在神经科学研究和先进的大脑计算机接口的开发中具有潜在的应用.
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