由Drosophila基因毛的子宫外表达引起的模式异常与Ftz转录抑制有关
1Department of Zoology, University of Oxford, England.
Cell
|November 6, 1987
概括
毛发 (h) 基因在多虫发育过程中起到转录抑制作用. 宫外h表达抑制fushi tarazu (ftz) 并影响其他细分基因,影响胚胎发育.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 配对规则基因毛 (h) 在Drosophila的胚胎细分中起着至关重要的作用.
- 在早期的Drosophila胚胎的特定部分 (metameres) 中表达和要求h.
研究的目的:
- 为了研究毛发 (h) 基因在多虫细分中的调控作用.
- 要确定h是否作为转录抑制剂及其下游目标.
主要方法:
- 使用hsp70促销器来驱动毛发 (h) 基因的宫外表达.
- 分析了子宫外h表达对fushi tarazu (ftz),engrailed (en) 和Ultrabithorax (Ubx) 的表达模式的影响.
主要成果:
- 宫外毛的 (h) 表达式被抑制的fushi tarazu (ftz) 在正常领域之外的表达.
- 动力学分析表明,h直接作为转录抑制剂.
- 观察到模式缺陷和改变en和Ubx活动,支持h在通过ftz和其他对规则基因建立en域中的作用.
- 提供了证据,证明 ftz 对于启动 Ubx 转录而不是维护至关重要.
结论:
- 毛 (h) 作为Drosophila细分中的直接转录抑制剂.
- 毛发 (h) 通过调节fushi tarazu (ftz) 和其他对规则基因来影响嵌 (en) 域的建立.
- 富士塔拉祖 (ftz) 对于启动,但不是维护Ultrabithorax (Ubx) 转录至关重要.
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