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Updated: Aug 11, 2025

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同位素标记原生梅利丁的重组表达和化学化

Martin D Gelenter1, Ad Bax1

  • 1Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0520, United States.

Journal of the American Chemical Society
|February 8, 2023
PubMed
概括

研究人员开发了一种使用重组表达和化学化生产本地梅利丁的方法. 这使得这个重要的生物分子的结构研究更加精确.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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