在体外5SRNA基因表达的差异化.
1Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210.
Cell
|December 4, 1987
概括
转录因子IIIA (TFIIIA) 度决定了Xenopus蛋提取物中的5SRNA基因转录. 降低TFIIIA水平可以增强体质5SRNA基因对卵细胞基因的转录,从而揭示出差异性基因调节机制.
科学领域:
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
- 基因规则 基因规则
背景情况:
- 在Xenopus中的卵细胞和体质5SRNA基因转录复合体在激活卵子提取物中表现出差异性稳定性.
- 转录因子IIIA (TFIIIA) 是5SRNA基因转录中的一个关键调节器.
研究的目的:
- 为了研究Xenopus蛋提取物中卵细胞和体质5SRNA基因转录复合物的差异稳定性.
- 确定转化作用因子,特别是TFIIIA在调节5SRNA基因转录中的作用.
主要方法:
- 使用活化Xenopus蛋的提取物.
- 操纵蛋提取物中的TFIIIA度.
- 对比克隆体和卵细胞5SRNA基因的转录效率.
主要成果:
- 卵细胞5SRNA基因转录复合体在激活的卵子提取物中是不稳定的,与体质复合体不同.
- 在不稳定复合体中,转化作用因子与自由因子存在平衡,使转录依赖于度.
- 与卵子基因相比,减少卵子提取物中的TFIIIA水平导致克隆体质5SRNA基因的转录增加了多达400倍.
结论:
- TFIIIA度是Xenopus蛋提取物中5SRNA基因转录的关键限制因素.
- 卵细胞和体质5SRNA基因的不同调节受到TFIIIA可用性和复合体稳定性的影响.
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