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Updated: Aug 11, 2026

The MultiBac Protein Complex Production Platform at the EMBL

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证明一种化学合成的BPV1coprotein及其C端域功能诱导细胞DNA合成

M Green¹, P M Loewenstein

¹Institute for Molecular Virology, St. Louis University School of Medicine, Missouri 63110.

|December 4, 1987

概括

牛乳头瘤病毒1型

科学领域:

在瘤学瘤学.
病毒学病毒学
分子生物学分子生物学

背景情况:

牛乳头瘤病毒1型 (BPV-1) 拥有已知最小的瘤基因,即开放阅读框架E5 (ORF E5).
该ORF E5编码一种疏水性44氨基酸蛋白质,E5蛋白,涉及到细胞转化.

研究的目的:

为了研究BPV-1 E5上蛋白的生物化学功能.
确定负责E5蛋白诱导细胞DNA合成的能力的特定域.

主要方法:

全长E5蛋白和各种删除突变的化学合成.
合成的微注射到增长停止的细胞中,以评估它们的生物活性.

主要成果:

E5 蛋白诱导细胞增长停止的细胞中的细胞DNA合成.
这一活动是由两个不同的功能领域介导的.
一个13氨基酸C端片段足以自主诱导DNA合成,代表已知最小的此类片段.

结论:

在BPV-1 E5上蛋白的C端13氨基酸构成一个最小的域,能够诱导细胞DNA合成.
疏水性中间区域显著增强了E5上蛋白的特定活性.
对于诱导DNA合成而言,N端区域并不必不可少.

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相关概念视频

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.