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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Aug 9, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

使用Aptamer启用近距离催化标记的细胞选择性多功能表面共价重构

Yuna Guo^1,2, Nan Wang¹, Yihong Zhong¹

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Journal of the American Chemical Society

|February 23, 2023

概括

研究人员开发了一种新的细胞表面工程平台, 这种方法可以同时安装多个分子,允许复杂的细胞编程和混合种群的跟踪.

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Published on: September 23, 2013

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

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科学领域:

生物技术
化学生物学
细胞生物学

背景情况:

细胞表面工程对于创建具有特定功能的自定义细胞接口至关重要.
现有的细胞选择性共价标签方法难以同时引入多个不同的功能分子.

研究的目的:

为多功能细胞表面重新配置开发一个能够使用aptamer的近距离催化共价标签平台.
在混合种群中的目标细胞上选择性同时安装多个功能分子.

主要方法:

过氧化酶与细胞选择性体的结合,以创建有针对性的标记探针.
使用近距离诱导的催化剂对目标细胞进行特定的共价标记.
使用改功能分子进行多种细胞表面操作和多重标记.

主要成果:

在混合种群中对目标细胞进行选择性共价标记.
通过同时安装两个直角点击反应分子,实现了多重对应标记.
在活细胞上成功进行了超快速新糖化,使得随后的糖重塑成为可能.

结论:

支持aptamer的平台为多功能细胞表面工程提供了多功能工具.
这种方法可以精确控制复杂生物系统中的细胞相互作用和表面重塑.
该平台为细胞选择性葡萄糖工程提供了一种新方法.