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使用Aptamer启用近距离催化标记的细胞选择性多功能表面共价重构

Yuna Guo^1,2, Nan Wang¹, Yihong Zhong¹

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Journal of the American Chemical Society

|February 23, 2023

概括

研究人员开发了一种新的细胞表面工程平台, 这种方法可以同时安装多个分子,允许复杂的细胞编程和混合种群的跟踪.

科学领域:

生物技术
化学生物学
细胞生物学

背景情况:

细胞表面工程对于创建具有特定功能的自定义细胞接口至关重要.
现有的细胞选择性共价标签方法难以同时引入多个不同的功能分子.

研究的目的:

为多功能细胞表面重新配置开发一个能够使用aptamer的近距离催化共价标签平台.
在混合种群中的目标细胞上选择性同时安装多个功能分子.

主要方法:

过氧化酶与细胞选择性体的结合,以创建有针对性的标记探针.
使用近距离诱导的催化剂对目标细胞进行特定的共价标记.
使用改功能分子进行多种细胞表面操作和多重标记.

主要成果:

在混合种群中对目标细胞进行选择性共价标记.
通过同时安装两个直角点击反应分子,实现了多重对应标记.
在活细胞上成功进行了超快速新糖化,使得随后的糖重塑成为可能.

结论:

支持aptamer的平台为多功能细胞表面工程提供了多功能工具.
这种方法可以精确控制复杂生物系统中的细胞相互作用和表面重塑.
该平台为细胞选择性葡萄糖工程提供了一种新方法.

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