基于海米的模块化支架的"四合一"设计,用于高对比度可激活的分子光照成像
Yongchao Liu1, Lili Teng1, Xiao-Feng Lou1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
Journal of the American Chemical Society
|February 24, 2023
概括
新的分子支架可以激活后发光成像,克服纳米粒子探测器的局限性. 这些探针可提升药物引起的肝毒性等疾病的早期检测灵敏度.
科学领域:
- 有机化学
- 生物医学成像
- 摄影化学
背景情况:
- 后发光 (长时间持续发光) 是分子成像的前景.
- 现有的余光探测器主要是纳米粒子,报告的有机小分子系统很少.
- 由于有限的反应点和通用支架,设计可激活的后照探针具有挑战性.
研究的目的:
- 开发基于半氨酸的新型分子支架,用于响应刺激的后光发光.
- 为多功能分子成像创建模块化可激活分子后发光探针 (MAP).
- 展示MAP在生物目标和疾病模型中的应用.
主要方法:
- 设计的"四合一"MAP集成刺激响应,1O2生成,1O2捕获和发光单元.
- 研究了用于光能存储和延迟发光的分子内级联光反应.
- 应用MAP用于pH,超氧化离子和氨基酶的量化和成像.
- 使用MAP用于药物诱导的肝毒性高对比度光照成像.
主要成果:
- 开发出基于半氨酸的支架,表现出对刺激有反应的光.
- 与光成像相比,MAP在光后成像中表现出更高的灵敏度.
- 成功成像目标包括pH,超氧化和氨基酶.
- 通过检测序列性氧化应激和氨基酶上调来实现药物诱导肝毒性早期的非侵入性光照成像.
结论:
- 新的分子支架为可激活的光后探测器提供了一个新的平台.
- MAP为分子成像应用提供了增强的灵敏度和多功能性.
- 可激活的光探针对肝毒性和其他疾病的早期诊断具有显著的前景.
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