在DExH-Box螺旋酶中进行RNA驱动的ATP水解的分子基础
Santiago Movilla1, Maite Roca1, Vicent Moliner1
1BioComp Group, Institute of Advanced Materials (INAM), Universitat Jaume I, 12071 Castellón, Spain.
Journal of the American Chemical Society
|March 17, 2023
概括
结合体
科学领域:
- 分子生物学
- 生物化学
- 结构生物学
背景情况:
- 拼接酶是一种大型分子机器,
- 依赖RNA的ATPases/helicases重塑了结合体,但它们的功能由RNA驱动的调节尚不清楚.
- Prp2 ATPase/helicase对于结合体的激活至关重要.
研究的目的:
- 阐明Prp2中RNA驱动的ATPase激活的分子机制.
- 了解RNA结合如何调节Prp2的酶活性.
主要方法:
- 古典和量子古典分子动力学模拟.
- 控制ATP水解和RNA结合的分子相互作用的剖析.
主要成果:
- 通过形成RNA结合道和催化部位之间的相互作用,RNA结合以异质方式激活Prp2的ATPase功能.
- 这种激活将关键残留物定位为有效的ATP水解.
- 确定了将RNA与ATPase/helicase活性结合的保存结构动机.
结论:
- 由RNA激活Prp2 ATPase/helicase的详细分子机制已经被发现.
- 这种机制涉及通过RNA结合的全调节,很可能在DEXH盒酶超级家族中保持.
相关概念视频
DNA Helicases
21.7K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.7K
Mechanical Protein Functions
5.0K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.0K
DNA Topoisomerases
31.5K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
31.5K
Restarting Stalled Replication Forks
5.9K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.9K
Homologous Recombination
50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K
Translesion DNA Polymerases
10.1K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.1K


