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Updated: Jun 10, 2026

Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
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在单分子分辨率下CFTR功能,病理和药理学

Jesper Levring1, Daniel S Terry2, Zeliha Kilic2

  • 1Laboratory of Membrane Biology and Biophysics, The Rockefeller University, New York, NY, USA.

Nature
|March 23, 2023
PubMed
概括

囊性纤维化跨膜导电调节器 (CFTR) NBD 在打开之前进行二元化,控制化物流动. 这种机制解释了CFTR增强剂的作用,并为新的囊性纤维化疗法提供了信息.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...