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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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现场和实时可视化双网络水凝中的机械化学损伤,通过氧释放激素捕获的前光探针

Yong Zheng1, Julong Jiang2, Mingoo Jin1,3

  • 1Institute for Chemical Reaction Design and Discovery (WPI-ICReDD), Hokkaido University, Sapporo 001-0021, Japan.

Journal of the American Chemical Society
|March 23, 2023
PubMed
概括

这项研究引入了一种使用光前探针和氧气可视化水凝中的分子规模损伤的新方法. 这种技术可以实时跟踪聚合物材料的机械化学损伤.

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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