Jove
Visualize
联系我们

相关概念视频

Mismatch Repair01:36

Mismatch Repair

Overview
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Expression of chemokines in Escherichia coli.

Methods in molecular biology (Clifton, N.J.)·2000
Same author

Recombinant guinea pig and human RANTES activate macrophages but not eosinophils in the guinea pig.

Journal of immunology (Baltimore, Md. : 1950)·1997
Same author

Purification, characterization, and in vitro phosphorylation of the neuron-specific membrane-associated protein SCG10.

Protein expression and purification·1997
Same author

A bacterial signal peptide directs efficient secretion of eukaryotic proteins in the baculovirus expression system.

Protein expression and purification·1997
Same author

Structure and bioactivity of recombinant human CTAP-III and NAP-2.

Journal of protein chemistry·1997
Same author

Dissecting processing and apoptotic activity of a cysteine protease by mutant analysis.

The Journal of cell biology·1996
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 26, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Mu插入在宿主插入位置重复一个5个基对序列.

B Allet

    Cell
    |January 1, 1979
    PubMed
    概括

    菌体Mu lysogenization涉及到在宿主DNA中的插入部位的五个基对的重复. 这导致直接重复围绕Mu DNA,类似于插入序列 (IS) 元素行为.

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 微生物学 微生物学

    背景情况:

    • lysogenic 菌体将其 DNA 整合到宿主基因组中.
    • 了解菌体Mu集成的机制对于分子生物学至关重要.
    • 之前的研究已经研究了DNA集成,但缺乏在插入部位的详细序列分析.

    研究的目的:

    • 分析 lysogenic Mu DNA 的整合部位上的核酸序列.
    • 为了确定Mu DNA集成是否会导致宿主DNA序列的改变.
    • 将Mu的整合机制与其他移动遗传元素进行比较.

    主要方法:

    • 对 lysogenic Mu DNA 两端核酸序列的分析.
    • 克隆Mu DNA的结果是lambda Mu杂交粒子.
    • 在lambdaplac5的 lacZ区域内使用Mu lysogen进行分析.

    主要成果:

    • lysogenization 与插入部位的 5 个基对 lac DNA 的重复有关.
    • 集成的MuDNA被这五个基对的两个副本围绕着,以直接重复为导向.
    • 独立的调查证实了这些发现,使用不同的Mu lysogen.

    更多相关视频

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations
    04:52

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations

    Published on: February 3, 2023

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
    07:28

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

    Published on: January 10, 2025

    相关实验视频

    Last Updated: Jun 26, 2026

    Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
    08:19

    Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

    Published on: July 7, 2020

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations
    04:52

    Following the Dynamics of Structural Variants in Experimentally Evolved Populations

    Published on: February 3, 2023

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
    07:28

    Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

    Published on: January 10, 2025

    结论:

    • 菌体 Mu 集成涉及到目标部位的 5 个基对重复,创建直接的重复.
    • Mu DNA 插入机制与插入序列 (IS) 介导的 DNA 插入有相似之处.
    • 这些发现提供了对病毒DNA整合和基因组进化的分子机制的见解.