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基于Avidity的方法来有效生成单基因的重组蛋白

Spencer L Nelson1, Yunan Li2, Yue Chen2

  • 1Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093, United States.

Journal of the American Chemical Society
|April 3, 2023
PubMed
概括

一种新方法可以大规模生产单化蛋白质,揭示了这种修饰对帕金森和ESCRT蛋白质的蛋白质聚合有相反的影响.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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