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在活细胞和斑马鱼中检测可逆识别酸探针

Kai Wang^1,2, Ruixiao Zhang³, Xiujie Zhao³

¹Institute of Materia Medica, Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong 250062, People's Republic of China.

Journal of the American Chemical Society

|April 6, 2023

概括

新的二博酸探针能够敏感地检测葡萄糖,有助于了解葡萄糖平衡和相关疾病. 这些探针可视化细胞和斑马鱼的葡萄糖差异, 提供强大的诊断工具.

科学领域:

生物医学工程
分析化学
分子成像

背景情况:

葡萄糖平衡对人类健康至关重要,但由于缺乏有效的成像探测器,人们对其了解甚少.
目前的成像技术不足以对葡萄糖水平波动进行详细分析.

研究的目的:

开发新型水溶性二博酸探针,用于敏感的葡萄糖检测.
使用斑马鱼模型对正常与瘤细胞的葡萄糖异质性进行研究.
建立一个新的策略来设计基于酸的先进葡萄糖探针.

主要方法:

合成二酸 (PDBA) 衍生物,Mc-CDBA和Ca-CDBA,包括水溶性和增强亲和力的组.
探测器灵敏度,检测极限和葡萄糖亲和力的表征.
在正常和瘤细胞中区分葡萄糖水平的应用.
在斑马鱼体内的葡萄糖成像使用Mc-CDBA和Ca-CDBA.

主要成果:

开发了具有高灵敏度 (F/F0=47.8,LOD=1.37μM) 和强的葡萄糖亲和度 (Ka=4.5×10^3M^-1) 的水溶性探针Mc-CDBA和Ca-CDBA.
通过使用Mc-CDBA成功确定了正常和瘤细胞之间的葡萄糖异质性.
用开发的探针在斑马鱼体内有效的葡萄糖成像.

结论:

新的二酸探针在葡萄糖检测和成像方面取得了重大进展.
这些探测器为评估与葡萄糖相关的疾病和理解新陈代谢过程提供了强大的工具.
这项研究提出了设计灵敏和特异性的酸葡萄糖探针的新策略.

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