使用光核酸对无细胞系统进行精确的正角遥控
Giacomo Mazzotti1, Denis Hartmann1, Michael J Booth1,2
1Department of Chemistry, University of Oxford, Mansfield Road, OX1 3TA Oxford, U.K.
Journal of the American Chemical Society
|April 19, 2023
概括
科学家们开发出新的光控制开关, 合成生物学的这一突破为医学和纳米技术的先进应用提供了远程的非侵入性控制.
科学领域:
- 合成生物学
- 生物技术
- 分子生物学
背景情况:
- 无细胞基因表达对纳米技术和合成生物学至关重要.
- 使用光精确,非侵入性控制无细胞系统是非常理想的,但具有挑战性.
- 对于无细胞表达的光控制OFF开关的开发已经落后于ON开关.
研究的目的:
- 开发直角光控制的无细胞表达OFF开关.
- 在无细胞系统中实现基因表达的精确,远程和非侵入性调节.
- 将光控制的关闭开关与现有的启动开关集成为动态控制.
主要方法:
- 将基和氨酸光连接到反感性寡核酸,以创建光控制的关闭开关.
- 使用商业可用的寡核酸进行交换机合成.
- 使用不同的光波长证明直角mRNA降解.
- 将开发的OFF开关与以前建立的蓝光激活的DNA模板ON开关结合起来.
主要成果:
- 成功开发了直角光控制的无细胞表达OFF开关.
- 实现了对细胞自由表达的严格控制.
- 证明了两个不同的mRNA的波长依赖的正交降解.
- 展示了使用不同光波长的转录 (ON) 和翻译 (OFF) 的顺序控制.
结论:
- 开发的光抗意义寡核化物提供了有效的,直角的光控制的OFF开关,以实现无细胞表达.
- 这项技术可以在无细胞系统中精确,远程和动态地启动/关闭基因表达.
- 这项进展对于合成生物学,生物逻辑门和合成细胞的应用具有重要意义.
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